These guidelines summarize the views of the European Association of Nuclear Medicine Neuroimaging Committee (ENC). The purpose of the guidelines is to assist nuclear medicine practitioners in recommending, performing, interpreting, and reporting the results of brain perfusion SPECT studies using 99mTc-labelled radiopharmaceuticals. The aim is to achieve a high quality standard of brain perfusion SPECT imaging, which can increase the diagnostic impact of this technique in neurological and psychiatric practice.