Manuals and protocols

Spreadsheet for Designing Aedes Mosquito Mass-Rearing and Release Facilities Version 1.0

Manual and Spreadsheet for Assessment of Sterile Insect Release Densities

Plan de Acción en Caso de Detección de Moscas de la Fruta No-Nativas Reguladas del Género Bactrocera spp En América Latina y El Caribe

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency (IAEA) and Organismo Internacional Regional de Sanidad Agropecuaria (OIRSA). Vienna, Austria (May 2019).

Fruit sampling guidelines for area-wide fruit fly programmes

These guidelines are aimed at facilitating the transfer of harmonized sampling procedures to National Plant Protection Organizations and horticultural industry of FAO and IAEA Member States that want to apply fruit sampling procedures for fruit fly population survey in area-wide action programmes. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (June 2019).

Standard Operating Procedures for Identification of Tsetse Species from Wild Populations and Laboratory Colonies

Guidelines for Colonization of Aedes Mosquito Species - Version 1.0.

This document aims to provide a description of procedures required for the establishment of Aedes aegypti and Ae. albopictus colonies in your insectary or laboratory. It presents a summary of the necessary steps such as collecting material from the field, species identification, and adapting your wild colony to laboratory conditions and artificial rearing procedures. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (May 2018).

Update of the technical leaflet released in six languages on the discrimination between two invasive Bactrocera fruit fly pests

Bactrocera zonata (peach fruit fly) sometimes overlaps in terms of distribution with Bactrocera dorsalis (Oriental fruit fly). A technical leaflet to help to discriminate among these two invasive pest species, was updated and produced in Arabic, Chinese, English, French, Portuguese, and Spanish. The leaflet will be useful for countries and regions where these species are present or at risk of incursion helping to implement the recommended emergency response and is now available to regulatory agencies, surveillance and identification staff, growers of horticultural products and other stakeholders. It was prepared by Marc De Meyer (Royal Museum for Central Africa, Belgium) and Ian White (formerly research associate Natural History Museum, UK).

“The Dashboard” for Managers of Sterile Insect Technique Production Facilities

Mosquitoes SIT trifold

The document contains in a nutshell a description of SIT use against mosquitoes from mass rearing to sterilization and release. It also contains basic elements necessary for the cost-effective application of area-wide SIT technology against mosquitoes vectors of disease. Vienna, Austria (April 2018).

Manual para diferenciar moscas de Anastrepha ludens (Loew) silvestres

This manual includes standardised and updated procedures to determine the fertility or sterility of irradiated adults of wild and genetic sexing Anastrepha ludens strains. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (January 2018).

Trapping Guideline for Area-Wide Fruit Fly Programmes

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (January 2018)

Fruit Sampling Guidelines for Area-Wide Fruit Fly Programmes (2017)

Guideline for packing, shipping, holding and release of sterile flies in area-wide fruit fly control programmes

Guidelines for mature tsetse sterile male pupae packaging for long distance shipment

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (May 2017).

Guidelines for routine colony maintenance of Aedes mosquito species - Version 1.0

This guideline aims to provide a description of procedures required for Ae. aegypti and Ae. albopictus colony routine rearing. It is a summary of necessary steps such as optimizing climatic conditions in the insectary, egg hatching, larval rearing, pupal and larval sorting, sugar and blood feeding, egg collection, handling and storage, used at the FAO/IAEA Insect Pest Control Laboratory (IPCL) to build and maintain a lab colony. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (November 2017).

Guidelines for standardised mass rearing of Anopheles mosquitoes - Version 1.0

The guideline provides a description of all procedures required for the mass-rearing of An. arabiensis using these newly designed adult cage and larval rearing unit, with a step-by-step guide to establishing, up-scaling and maintaining a large Anopheles colony, including all stages of the mosquito’s life cycle. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (October 2017).

Technical specification for an X-ray system for the irradiation of insects for the sterile insect technique and other related technologies

Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (August 2017).

Manual to differentiate wild mediterranean fruit flies Ceratitis capitata (Wied.) from non-irradiated (fertile) and irradiated (sterile) Vienna temperature sensitive lethal strain flies

This manual includes standardised and updated procedures to determine the fertility or sterility of adults of the VIENNA Mediterranean fruit fly temperature sensitive lethal (TSL) strains. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (June 2016). ISBN 978-92-5-109055-8.

Standard operating procedure manual for sterile Tsetse release

This manual describes the standard procedures involved in preparing tsetse flies reared in a breeding facility for release in the field for the sterile insect technique (SIT). Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria (November 2016).

The use of mathematics in area-wide integrated pest management programmes using the sterile insect technique with a special focus on Tephritid fruit flies

Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, (September 2016). ISBN 978-92-5-109190-6.