Isothermal conditions used for LAMP are usually between 60 to 65°C, with an optimum of 62.5°C. However, in our tests performed between 55 to 65°C it was found that optimum temperatures for both Bsm and Bst was below 60°C and gave faster results than published positive results for AI-RT-LAMP (optimum around 57°C).
Applying the same reagents and concentrations a PPR-LAMP was performed on vaccine c-DNA using primers designed by Dr. Gang Li, Chinese Academy of Agricultural Sciences. It was found that the reaction worked extremely similar to the AI test. The results obtained with the AI LAMP give promise that this procedure can be further developed into a rapid and quality assured diagnostic system. The EVA green based platform will help to better evaluate these tests with established laboratory equipment in our counterpart laboratories for the fitness for purpose and their future field application.